Inducing and quantifying forbidden reactivity with single-molecule polymer mechanochemistry

Polymer mechanochemistry: Flex, release and repeat.

Model systems for single molecule polymer dynamics.

Double stranded DNA (dsDNA) has long served as a model system for single molecule polymer dynamics. However, dsDNA is a semiflexible polymer, and the structural rigidity of the DNA double helix gives rise to local molecular properties and chain dynamics that differ from flexible chains, including synthetic organic polymers. Recently, we developed single stranded DNA (ssDNA) as a new model syste...

Dendronized polymer is a Single Molecule Glass

Submitted for the MAR05 Meeting of The American Physical Society Dendronized polymer is a Single Molecule Glass JAYAJIT DAS, Dept. of Chemical Engr., UC Berkeley, YOSHIDA MASARU, ZACHARY FRESCO, TAE-LIM CHOI, JEAN FRECHET, Dept. of Chemistry, UC Berkeley, ARUP CHAKRABORTY, Dept. of Chemical Engr., UC Berkeley — The molecular architecture of dendronized polymers can be tuned to obtain nanoscale ...

Single-molecule tracking of polymer surface diffusion.

The dynamics of polymers adsorbed to a solid surface are important in thin-film formation, adhesion phenomena, and biosensing applications, but they are still poorly understood. Here we present tracking data that follow the dynamics of isolated poly(ethylene glycol) chains adsorbed at a hydrophobic solid-liquid interface. We found that molecules moved on the surface via a continuous-time random...

Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells.

Protein and messenger RNA (mRNA) copy numbers vary from cell to cell in isogenic bacterial populations. However, these molecules often exist in low copy numbers and are difficult to detect in single cells. We carried out quantitative system-wide analyses of protein and mRNA expression in individual cells with single-molecule sensitivity using a newly constructed yellow fluorescent protein fusio...